The name of the woman, described as 53 years old and white, has not been released pending notification of next of kin.
Police responded to the woman's home at 2630 13th Avenue North about 8:15 p.m. on a welfare check after a neighbor was concerned that she had been acting strangely all day, said Bill Proffitt, spokesman for the St. Petersburg Police Department.
The woman appeared to have mental problems, the neighbor told police, Proffitt said.
Two officers knocked on the front door and announced their presence. No one responded.
Meanwhile, a back-up uniformed officer went to the rear of the home and knocked on the back door.
The woman stood inside of the home, near the back door, and pulled back the curtain on a large window adjacent to the rear door and pointed a .38 revolver at the officer.
The officer fired his handgun three times through the window, striking the woman possibly twice in the chest area.
She was transported to Bayfront Medical Center where she died a short time later.
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St. Petersburg woman killed after pointing gun at officer